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CX3CR1-GFP

CX3CR1-GFP

With the endogenous Cx3cr1 locus, CX3CR1-GFP mutant can have detectable EGFP expression in brain microglia, dendritic cells, NK cells, and monocytes so that those cells can be visualized and traced.

As even the homozygous mutant are fertile, viable with normal size, and exhibit no gross physical or behavioral abnormalities. Instead of a disease model, CX3CR1-GFP might be better considered as a tool model for the investigation of leukocyte migration, trafficking and transplantation.

Add Animal Model Information


Genetics

  • Under the control of endogenous Cx3cr1 locus, homozygous EGFP is expressed in CX3CR1-GFP mice in brain microglia, dendritic cells, NK cells, and monocytes.(The Jackson Laboratory)
  • Homozygous CX3CR1-GFP merely express the mutant but no wild type gene product, as evident in RT-PCR analysis of lymphoid tissue and flow cytometric analysis of peripheral blood cells.(The Jackson Laboratory)
  • Approximate Controls: C57BL/6J(The Jackson Laboratory)

Application

  • In brain microglia, dendritic cells, NK cells, and monocytes, Enhanced Green Fluorescent Protein (EGFP) is expressed to mimick the endogenous gene expression.(The Jackson Laboratory)
  • The EGFP levels are detectable even in the peripheral blood cells in heterozygote mice(The Jackson Laboratory)
  • Therefore, CX3CR1-GFP mice, carrying the mutation which can add the detectable EGFP to various leukocyte, might be suitable for the investigation of leukocyte migration, trafficking and transplantation.(The Jackson Laboratory)
  • Note: GFP expression is downregulated in monocytes when they differentiate into macrophages.(The Jackson Laboratory)

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